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產(chǎn)品名稱(chēng):Human IFN-gamma

產(chǎn)品型號:SEKH-0046

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產(chǎn)品特點(diǎn):Human IFN-gamma索萊寶ELISA試劑盒的優(yōu)勢:
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SEKH-0046Human IFN-gamma的詳細資料:

Human IFN-gamma 

中文名稱(chēng)人干擾素γ檢測試劑盒
英文名稱(chēng)Human IFN-γ ELISA KIT
ReactivityHuman
Sample typeSerum, plasma, Cell culture supernatant
Sensitivity4 pg/ml
Detection TargetIFN-γ
ApplicationsSandwich ELISA

Human IFN-gamma

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is captured by the coated antibody after incubation. Following extensive washing, a biotin-conjugate antibody specific for IFN-γ is added to detect the captured IFN-γ protein in sample. For signal development, horseradish peroxidase (HRP)-conjugated Streptavidin is added, followed by Tetramethyl-benzidine (TMB) reagent. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm

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